magnetic activated cell sorting

Magnetic-activated cell sorting - Wikipedia

Magnetic-activated cell sorting (MACS) is a method for separation of various cell populations depending on their surface antigens (CD molecules) invented by Miltenyi Biotec. The name MACS is a registered trademark of the company. The method is performed using Miltenyi Biotec's MACS Technology, which uses...

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Magnetic activated cell sorting: technology and advantages - Sepmag

Dec 21, 2017 ... Magnetic activated cell sorting is a very efficient process to separate certain cells from a population. Learn the advantages of this technology.

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MACS Cell Separation - Miltenyi Biotec

Magnetic cell isolation by Miltenyi Biotec is the proven high-quality method of cell separation (22000+ publications) - explore & learn more.

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Magnetic activated cell sorting (MACS)--a new immunomagnetic ...

Eur J Haematol. 1994 May;52(5):267-75. Magnetic activated cell sorting (MACS)--a new immunomagnetic method for megakaryocytic cell isolation: comparison of different separation techniques. Schmitz B(1), Radbruch A, Kümmel T, Wickenhauser C, Korb H, Hansmann ML, Thiele J, Fischer R. Author information:

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Magnetic-activated cell sorting (MACS) can be used as a large-scale ...

Jan 22, 2015 ... Here we show that magnetic-activated cell sorting (MACS) can be used for the large-scale generation of neuronal-restricted progenitor (NRP) cultures from embryonic zebrafish. Our findings provide a simple and semi-automated method that is likely to boost the use of neuronal cell cultures as a tool for the...

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Fluorescence- and magnetic-activated cell sorting strategies to ...

Nov 18, 2016 ... In recent years, the immune-magnetic bead-based system, namely MACS (Magnetic-activated cell sorting), has been widely used for cell separation. Based on immune-magnetic beads coupled with antibodies against sperm-specific antigens, MACS is fast, easy and economic. With a series of sperm...

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Magnetic-activated cell sorting - an overview | ScienceDirect Topics

Magnetic activated cell sorting (MACS) captures CTC by immunolabeling superparamagnetic particles (~ 50 nm diameter) (Miltenyi Biotec, Germany). These beads are composed of a biodegradable matrix. It is therefore not necessary to remove them from cells after the separation process. Isolated cells are preserved in...

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MACS (Magnetic Activated Cell Sorting). - YouTube

Feb 7, 2016 ... MACS MACS: does this procedure really help sperm quality? ICSI (Intracytoplasmatic sperm injection) or microinjection was revolutionary in IVF treatment. It ...

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efficiency of magnetic-activated cell sorting and fluorescence ...

Nov 2, 2006 ... Cell sorting by magnetic-activated cell sorting (MACS) and/or fluorescence-activated cell sorting (FACS) may be a solution to obtain both enrichment and decontamination. This study, therefore, aimed at evaluating the decontamination potential of these two cell sorting techniques for both murine and human...

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Magnetic-Activated Cell Sorting (MACS) | Service | Flow Cytometry ...

MACS® Technology is a fast and gentle method for the isolation of viable and functionally active cells by magnetic labeling. Magnetic-Activated Cell Sorting (MACS). Instruments · Applications · Protocols · To top · Impressum, Contact and Legal Notice · Contact page editor; Print page. Facebook · Twitter · YouTube · iTunes U...

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Generation of iPSCs as a Pooled Culture Using Magnetic Activated ...

Aug 17, 2015 ... To develop higher throughput iPSC reprogramming methods, we generated iPSCs as a pooled culture using rigorous cell surface pluripotent marker selection with TRA-1-60 or SSEA4 antibodies followed by Magnetic Activated Cell Sorting (MACS). We observed that pool-selected cells are similar or...

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Magnetic activated cell sorting (MACS): Utility in ... - Semantic Scholar

Magnetic activated cell sorting (MACS) is a novel sperm preparation technique that separates apoptotic and non-apoptotic spermatozoa based on the expression of phosphatidylserine. This has led to the incorporation of MACS as a sperm preparation technique. The review highlights the principle and mechanism of this...

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Male Factor Infertility Outcomes Using Magnetic Activated Cell ...

Following this rationale, Magnetic Activated Cell Sorting-MACS [22] has been applied as a sperm preparation method to remove apoptotic cells using Annexin V [8,23,24]. Overall, MACS is a method to separate cells of interest from a mixed cell population. Their principle is the use of magnetic micro beads conjugated with...

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Magnetic-Activated Cell Sorting (MACS) abroad: Clinics, Prices ...

Results 1 - 15 of 20 ... MEDIGO lists clinics around the world offering Magnetic-Activated Cell Sorting (MACS) procedures from $ 267. Save up to 80% on Reproductive Medicine treatment abroad.

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Evaluation of sperm recovery following annexin V magnetic ...

Magnetic-activated cell sorting (MACS) using paramagnetic annexin V-conjugated microbeads eliminates spermatozoa with externalized phosphatidylserine, which is considered one of the features of apoptosis. The objective of this study was to evaluate sperm recovery following the use of MACS as a sperm preparation...

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Is Magnetic-Activated Cell Sorting an Efficient Technique in ...

morphologically normal spermatozoa but do not necessarily select spermatozoa according to their functional capacity or genetic quality [2]. Therefore, Magnetic-Activated Cell Sorting (MACS) has been used in ART as a complement of the foregoing techniques. This technology relies on the detection of phosphatidylserine.

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Simultaneous sorting of multiple bacterial targets using integrated ...

Simultaneous sorting of multiple bacterial targets using integrated. Dielectrophoretic–Magnetic Activated Cell Sorter†. Unyoung Kima and H. Tom Soh*ab. Received 25th February 2009, Accepted 1st May 2009. First published as an Advance Article on the web 14th May 2009. DOI: 10.1039/b903950c. The ability to rapidly...

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Magnetic Activated Cell Sorting (MACS) - SlideShare

Mar 23, 2017 ... MACS is a sperm separation technique used in A.R.T. It allows separation of good quality sperm from apoptotic sperm (Sperm programmed to die) which may be one …

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Magnetic-activated cell sorting before ... - Springer Link

Superparamagnetic annexin-V conjugated microbeads are able to eliminate spermatozoa with externalized phosphatidylserine, a membrane feature of apoptotic cells as well as spermatozoa with deteriorated plasma membrane. Our objective was to evaluate the effects of annexin-V Magnetic-Activated Cell Sorting. (MACS)...

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MACS (magnetic activated cell sorting) - Sanatorium Helios

MACS (magnetic activated cell sorting). New, non-invasive method of selecting sperm according to its functional status. Thanks to MACS it is possible to modify sperm to increase the chances of successful fertilization in any method of assisted reproduction. Using MACS, we can collect sperm with damaged DNA and...

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Effects of magnetic-activated cell sorting on sperm ... - Cleveland Clinic

Objective: To evaluate the effect of magnetic-activated cell sorting in cryopreservation–thawing protocols on sperm motility and cryosurvival rate. Design: Prospective-controlled study. Setting: Andrology department at a university-based medical institution. Patient(s): Ten healthy volunteer sperm donors. Intervention(s):...

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New cell sorting technology could replace fluorescent and magnetic ...

This technique enables manipulation of non-magnetic micro- and nano-entities, including cells, immersed in magnetic fluids. ... Cell sorting could be used much more extensively, but the existing technologies, fluorescently activated cell sorting (FACS™) and magnetically activated cell sorting (MACS®), are expensive.

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Selection of nonapoptotic sperm by magnetic-activated cell sorting ...

In particular, magnetic-activated cell sorting is used to eliminate apoptotic spermatozoa. This study represents the proof-of-concept for magnetic-activated cell sorting applicability in teleost species relevant in aquaculture. Apoptotic cell population was studied by flow cytometry using YO-PRO-1 and a caspase detection kit.

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QμickBeads™: Magnetic isolation of rare stem cells via a capture ...

Magnet-activated cell sorting (MACS) is a well-developed and widely utilized method of isolating cells of interest in clinical medicine as well as basic bi.

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Magnetic Activated Cell Sorting performed before double gradient ...

The use of Magnetic Activated Cell Sorting (MACS) for seminal preparation in order to reduce apoptotic spermatozoa and improve clinical pregnancy rates after Assisted Reproduction Techniques (ART) has been considered. However, is not well established whether the use of MACS before or after the standard methods of...

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Magnetic Cell Sorting Is a Fast and Effective Method of ... - CiteSeerX

Magnetic Cell Sorting Is a Fast and Effective Method of Enriching Viable. Spermatogonia from Djungarian Hamster, Mouse, and Marmoset Monkey Testes1. Viktoria von Schönfeldt, Hanumanthappa Krishnamurthy, Luca Foppiani, and Stefan Schlatt2. Institute of Reproductive Medicine of the University Münster, 48149...

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FACS versus MACS for stem cell purification - Stem Cell Assays

Jan 12, 2013 ... Fluorescent-Activated Cell Sorting (FACS) or Magnetic-Activated Cell Sorting (MACS) are most frequently used methods for cell purification. We're routinely use both methods in research for purification of stem cell populations. We know that FACS can provide better purity and more.

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MACS (Magnetic Activated Cell Sorting) - Instituto Bernabeu

Oct 2, 2013 ... This has allowed a sperm selection technique called MACS (magnetic-activated cell sorting) to be designed. It uses the magnetic microparticles bound to a protein called annexin V that then bind to the markers, phosphatidylserine, that are found in the external part of the sperm membranes that are set for...

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Isolation of THY1+ Undifferentiated Spermatogonia from Mouse ...

To gain insight into germline stem cell biology and develop strategies for infertility treatment, several germ cell isolation methods have been reported in order to acquire good quality and quantity of undifferentiated spermatogonia. Among them, magnetic-activated cell sorting (MACS) is an efficient cell isolation method that...

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DNA fragmentation in human sperm after magnetic-activated cell ...

Nov 6, 2014 ... Purpose. As fertilization with unselected apoptotic spermatozoa may contribute to failures in assisted reproductive techniques, it has become essential to remove this type of sperm in order to increase the success rates. Magnetic-activated cell sorting (MACS) is a sperm preparation technique that isolates...

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ISOLATING CELLS FROM BLOOD USING BUOYANCY ACTIVATED ...

standard method in obtaining target cells from heterogeneous cell populations. This is conventionally done using fluores- cence-activated cell sorting (FACS), magnetic-activated cell sorting (MACS) techniques, and recently microfluidic sys- tems [8]. FACS can obtain highly purified cells, however, the high flow rate required...

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Multitarget Dielectrophoresis Activated Cell Sorter - UCSB Computer ...

Nov 26, 2008 ... Unfortunately, many current selection methods for cell separation, such as magnetic activated cell sorting (MACS), only allow the binary separation of target cells that have been labeled via a single parameter (e.g., magnetization). This limitation makes it challenging to simultaneously enrich multiple, distinct...

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Cell Purification: A New Challenge for Biobanks - FullText ...

Mar 16, 2015 ... Other antibody-mediated cell isolation methods such as fluorescence-activated cell sorting (FACS) and magnetic-activated cell sorting (MACS) are highly efficient cell purification techniques for the isolation of viable cells with a much higher purity, but both methods present several limitations. Since they do...

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MACS (Magnetic Activated Cell Sorting) | Reproducción Asistida

Técnica de preparación seminal diseñada para optimizar la selección de espermatozoides.

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MACS - Science Exchange

MACS - Science Exchange Lets You Compare Quotes From Leading Service Providers.

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Perspective on Microfluidic Cell Separation: A ... - ACS Publications

Oct 28, 2014 ... magnetic-activated cell sorting (FACS and MACS, respec- tively), to more specialized approaches based on affinity, dielectrophoretic mobility, and inertial properties of cells. With several of these technologies nearing commercialization, there is a sense that the field of microfluidic cell separation has...

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Magnetic Activated Cell Sorting: MACS - Med

Since the birth of Louise Brown, conceived using IVF, the evolution in ART has been remarkable. ❖ 15% of couples of reproductive age encounter fertility problems. ❖ This is solved, in part, by ARTs. ❖ Each single case is handled individually according to request and medical knowledge. ❖ There is still room for...

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Capturing the in vivo molecular signature of the podocyte - Kidney ...

Glomeruli were isolated from bitransgenic mice (mT/mG;hNPHS2Cre) by perfusion of magnetic particles and then were dissociated into a single-cell suspension before fluorescence-activated sorting of podocytes. The yield of extraction was greater than 5 × 105 podocytes per mouse, which was sufficient for transcriptome...

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Increased pregnancy after reduced male abstinence: Systems ...

Sep 20, 2013 ... Said, T.M., Agarwal, A., Grunewald, S., Rasch, M., Glander, H.J. and Paasch, U. (2006) Evaluation of sperm recovery following annexin V magnetic-activated cell sorting separation. Reprod Biomed Online 13:336–339. [Google Scholar]. Salian, R.S., Kalthur, G., Uppangala, S., Kumar, P. and Adiga, S.K....

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Andrology Lab Corner* Utility of Magnetic Cell ... - Cleveland Clinic

Nov 12, 2007 ... superparamagnetic microbeads (,50 nm in diameter) conjugated with annexin V bind to PS and are used to separate dead and apoptotic spermatozoa by magnetic-activated cell sorting (MACS). Cells with externalized PS will bind to these microbeads, whereas nonapoptotic cells with intact membranes do...

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